YAC Libraries A User's Guide |
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| Editor:
| Nelson, David L.
Brownstein, Bernard |
| Series title: | University of Wisconsin Biotechnology Center Biotechnical Resource Ser. |
| ISBN: | 978-0-7167-7014-5 |
| Publication Date: | Jan 1996 |
| Publisher: | Oxford University Press, Incorporated
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| Book Format: | Paperback |
| List Price: | AUD $90.00 |
Book Description:
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An indispensable guide to an emerging technology, this unique book introduces detailed methods for the construction, screening, and use of DNA sequences cloned into yeast artificial chromosome (YAC) vectors and the applications of YACs to gene hunting. Also covered are the reconstruction of large genes and the analysis of YACs by insertion back into mammalian cells--a method that may lead to the therapeutic use of YAC clones. The methods described are drawn form work on human DNA, but...
More DescriptionAn indispensable guide to an emerging technology, this unique book introduces detailed methods for the construction, screening, and use of DNA sequences cloned into yeast artificial chromosome (YAC) vectors and the applications of YACs to gene hunting. Also covered are the reconstruction of large genes and the analysis of YACs by insertion back into mammalian cells--a method that may lead to the therapeutic use of YAC clones. The methods described are drawn form work on human DNA, but are universal to yeast artificial chromosomes used in other organisms. YAC Libraries is the third volume in the UWBC Biotechnical Resource Series with Dr. Richard Burgess, Director of the University of Wisconsin Biotechnology center, as Series Editor. The series is dedicated to providing practical guidance to researchers exploring new technologies and services.